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Gnat Attack some cordycep militaris


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#41 whitethumb

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Posted 08 December 2016 - 01:25 AM

thanks for the update cat!!!
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#42 CatsAndBats

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Posted 08 December 2016 - 09:46 AM

 post-147940-0-34628000-1481208317.jpg

 

Jars at ~90% after 7 days with liquid inoculate.

Attached Thumbnails

  • cordy grain.jpg

Edited by catattack, 08 December 2016 - 09:46 AM.

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#43 CatsAndBats

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Posted 09 December 2016 - 09:54 AM

Oh yeah, @coorsmikey suggested that I use bat guano, I think it's a great idea. Purrhaps a BGBRF cake?

 

Oh yeah, I trashed the LCs, the jars and lids can be put to better use.


Edited by catattack, 09 December 2016 - 10:54 AM.


#44 CatsAndBats

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Posted 10 December 2016 - 07:10 PM

More links and PDFs on it's cultivation:

 

https://www.hindawi....wj/2014/510627/

 

Abstract

Cordycepin is one of the most important bioactive compounds produced by species of Cordyceps sensu lato, but it is hard to produce large amounts of this substance in industrial production. In this work, single factor design, Plackett-Burman design, and central composite design were employed to establish the key factors and identify optimal culture conditions which improved cordycepin production. Using these culture conditions, a maximum production of cordycepin was 2008.48 mg/L for 700 mL working volume in the 1000 mL glass jars and total content of cordycepin reached 1405.94 mg/bottle. This method provides an effective way for increasing the cordycepin production at a large scale. The strategies used in this study could have a wide application in other fermentation processes.

 

 

Download here entire PDF here:

 

Attached File  cordycep open source.pdf   6.24MB   12 downloads

 

Bonus download here (spent yeast grow):

 

Attached File  cordyceps spent brewers grain.pdf   360.27KB   11 downloads

 

 


Edited by catattack, 10 December 2016 - 07:14 PM.

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#45 CatsAndBats

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Posted 10 December 2016 - 07:25 PM

 post-147940-0-11268200-1481415740.jpg

 

Please note the continued yellowing of the mycelium! Looks promising IMHO.

 

post-147940-0-84487100-1481415779.jpg

 

Bonus pic of spider found here:

 

http://io9.gizmodo.c...to-grow-antlers

Attached Thumbnails

  • cordy bulk.jpg
  • cordys growing on spider carcass.jpg

Edited by catattack, 10 December 2016 - 07:26 PM.

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#46 Ramble

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Posted 10 December 2016 - 10:47 PM

Nice!!!
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#47 CatsAndBats

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Posted 11 December 2016 - 03:25 PM

post-147940-0-37663000-1481487871.jpg

 

 

 

post-147940-0-24229000-1481487876.jpg

 

 

These look like pins to me! Only time will tell.

Attached Thumbnails

  • CORDYCEPpins.jpg
  • CORDYCEPpins2.jpg

Edited by catattack, 11 December 2016 - 03:26 PM.

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#48 whitethumb

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Posted 11 December 2016 - 03:33 PM

awesome cat!!!
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#49 coorsmikey

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Posted 11 December 2016 - 03:40 PM

Looking Good! Makes me want to break out the Cordy Cultures. Is that the BGBRF sub in the tub?

#50 CatsAndBats

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Posted 11 December 2016 - 03:55 PM

Looking Good! Makes me want to break out the Cordy Cultures. Is that the BGBRF sub in the tub?

 

Naw man, that's my normal 50:50. I haven't broken out the guanos yet. I have bat and seabird guano. Still in the experimental stages. I wanted to see if I could be successful with normal mycology supplies, and then branch out to exotic subs or bug based subs. 


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#51 Needles

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Posted 11 December 2016 - 04:12 PM

They look like pins to me too. Exciting

#52 CatsAndBats

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Posted 11 December 2016 - 04:26 PM

So basically, if those do indeed fruit, we are halfway there. (living on a prayer)



#53 CatsAndBats

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Posted 12 December 2016 - 03:38 PM

I am going to try some popcorn with zero additives so that I can make food grade mycelium.



#54 Ramble

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Posted 12 December 2016 - 03:39 PM

Wooooooo!!!!
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#55 whitethumb

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Posted 12 December 2016 - 05:39 PM

what what... looks so promising!
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#56 Microbe

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Posted 12 December 2016 - 06:11 PM

They do look pins but they are not in the traditional sense. Inside that jar is about to look like whats growing out of the spider. I hear that the mummified bug that the Cordy fruits out of in nature contains a tremendously larger amount of antioxidants and other medicinal values.

You should attempt to print it by rupturing it. The problem is how will you know when has produced spores? Perhaps you can split it over some foil or other printing media and capture them?

Cordyceps loose vigor extremely quickly and in comparison to most other fungi we are familiar with, it is at light speed. It would be extremely difficult to isolate this because of that purpose but if you get a fruiting culture, they are just like other fungi they may fruit or may not it is just more common with cordyceps. My point there is along with printing. I dont think you want to clone this as more then likely it is already a clone but i may be wrong as it could have been expanded from a master culture.

None the less good work and im anxiously waiting to see it fruit.

This may be of interest to you.

http://www.omicsgrou...4.php?aid=73906
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#57 CatsAndBats

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Posted 13 December 2016 - 09:31 AM

They do look pins but they are not in the traditional sense. Inside that jar is about to look like whats growing out of the spider. I hear that the mummified bug that the Cordy fruits out of in nature contains a tremendously larger amount of antioxidants and other medicinal values.

You should attempt to print it by rupturing it. The problem is how will you know when has produced spores? Perhaps you can split it over some foil or other printing media and capture them?

Cordyceps loose vigor extremely quickly and in comparison to most other fungi we are familiar with, it is at light speed. It would be extremely difficult to isolate this because of that purpose but if you get a fruiting culture, they are just like other fungi they may fruit or may not it is just more common with cordyceps. My point there is along with printing. I dont think you want to clone this as more then likely it is already a clone but i may be wrong as it could have been expanded from a master culture.

None the less good work and im anxiously waiting to see it fruit.

This may be of interest to you.

http://www.omicsgrou...4.php?aid=73906

 

Appreciate the thoughts and information. Here's the downloadable version kids:

 

Attached File  the-strategies-for-increasing-cordycepin-production-of-cordyceps-militaris-byliquid-fermentation-2165-8056-1000134.pdf   416.28KB   9 downloads

 

I'm behind on my paper reading. I feel like a struggling teacher's assistant. :tongue:


Edited by catattack, 13 December 2016 - 09:31 AM.

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#58 CatsAndBats

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Posted 14 December 2016 - 10:10 AM

It's beginning to look a lot like CHRISTMAS!

 

God I hope these are fruits:

 

post-147940-0-04704300-1481728209.jpg

 

If they are, I'll adjust the recipe based on fruit size and the various cordycepin inducing papers that I've read/skimmed/posted.

 

 

Cordycepin molecule:

gallery_147940_1513_2240.png

Attached Thumbnails

  • cordy bin3.jpg

Edited by catattack, 14 December 2016 - 11:25 AM.

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#59 peptorepto

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Posted 14 December 2016 - 12:50 PM

Looks promising! Can't wait to see the results...


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#60 Ramble

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Posted 14 December 2016 - 05:54 PM

The suspense is killing me!!!






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