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Microbe's Randoms


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#1 Microbe

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Posted 31 October 2016 - 07:57 PM

This is going to be a bunch of random stuff i will be working on. Some very boring, some very interesting.

Lets start with a happy Halloween!

This picture was taken 2 years ago at my old house, damn i remember working in that hallway right beside my washer and dryer and behind me through that door is where the magic happened!

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About to do some flow bench work and while im doing that i decided to give my pp5 squeeze bottle agar container another go with several modifications. The PC is heating up right now with a test bottle in it with just water.

Remember when i tried this the first time the syringe filters popped off, the bottles floated around and melted? If not, they did!

So a went with a eazyfelt/micropore filter, and also used some tape around the lid, i dont trust it and even though this is just a test run, i want to mimic exactly how it be if i were cooking some agar.

I dropped the bottle in a wide mouth jar to keep it put once the water starts rolling, i cant believe i didnt even think about that my first run!

Sicks though, i had to bring out one of my 24's lmao

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Time to get to work!
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#2 Tenderfoot

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Posted 31 October 2016 - 08:07 PM

Work your show!!

 

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#3 Microbe

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Posted 31 October 2016 - 08:32 PM

Can a mod fix my spelling error? Change it from Micribe to Microbe?

Sent from my LGLS992 using Tapatalk
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#4 Microbe

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Posted 31 October 2016 - 08:34 PM

And here we go, failure again. I dont believe these are PP5 bit instead polyethylene! I thought 1.00 each was cheap for pp5, oh well.

Look at the dang high temp check valve, i just need to find the right squeeze container to put it on if one even exists. Although i pour just a fraction of the plates i used to, i still want a even more efficient way to pour. Back to the drawing board!

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Edited by Microbe77, 31 October 2016 - 09:06 PM.

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#5 Microbe

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Posted 31 October 2016 - 08:34 PM

Yeah i left my cable ties in there, didnt fill like messing with them....

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#6 Microbe

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Posted 31 October 2016 - 08:38 PM

Another disappointment, my syringe filters became loose during in the PC so i just ripped them all off, i should have known better again, these things needed to be JB Welded instead of using silicone.

The one left on the table with just the SHIP and filter, was my open and test the grain jar. I prepped these very dry, want to see just how dry i can go when making grain for expanding inoculate.


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#7 Microbe

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Posted 31 October 2016 - 08:43 PM

I did not open this one and inoculated with a very weak looking APE culture, the entire 60 mm plate. I cut the plate like a pizza and then dumped into the jar then shook.

Something that helps keep contamination away is to clean the threads when removing the lid. I will spin the jar while while holding a alcohol soaked paper towel that goes along the threads as i loosen the lid, its hard to explain. This is absolutely critical in G2G transfers.

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#8 Microbe

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Posted 31 October 2016 - 08:53 PM

Those of you who work with WBS can probably tell that this grain is very dry. Im experimenting to see just how dry i can make the grain but still support mycelia growth.

I steeped in the jar and after 1 hour all the water had been absorbed into the grain and literally the only reason why i had to strain the grains for a few was because i rinsed them.

I dont worry about sunflower seeds or what some call 'floaters' heck i have had oats with rodent remains and twigs in it and never had a problem.



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After adding water

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1 hour later and after a shake.

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You seen what it looked like after the PC in the previous post. I would never prepare my working spawn this way as it wouldnt be practical and would require many many jars but for testing and training of cultures and increasing inoculate, heck yeah.
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#9 wharfrat

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Posted 31 October 2016 - 09:01 PM

pulling up a chair.. and fixed title for ya  :biggrin:


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#10 Microbe

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Posted 31 October 2016 - 09:03 PM

pulling up a chair.. and fixed title for ya

Thanks!
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#11 hyphaenation

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Posted 01 November 2016 - 02:01 AM

Amazeballs! 

 

Fully impressed and mindblown. Thankee !



#12 whitethumb

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Posted 01 November 2016 - 08:44 AM

cool thread, looking forward to see your work!
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#13 Microbe

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Posted 01 November 2016 - 12:00 PM

Amazeballs!

Fully impressed and mindblown. Thankee !

Over melting plastic, a check valve that survived a pc and a few dry grain jars?

I thinkee you messee with memee!
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#14 hyphaenation

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Posted 01 November 2016 - 12:15 PM

I was stoned pretty heavy and I thought that was a clean air injector...but now I reread and realize its for agar , still very cool though.


Edited by hyphaenation, 01 November 2016 - 12:19 PM.

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#15 Hash_Man

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Posted 01 November 2016 - 03:44 PM

Weird microbe, almost seems like the cut/cracked corn and maybe the sunflower seed are the only ones to absorb moisture and the more viable seed (imo) not so much. . your such a Rebel : )
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#16 CatsAndBats

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Posted 03 November 2016 - 01:02 PM

 

Amazeballs!

Fully impressed and mindblown. Thankee !

Over melting plastic, a check valve that survived a pc and a few dry grain jars?

 

cutting edge.



#17 Microbe

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Posted 07 November 2016 - 04:18 PM

I should have taken a picture of this APE culture in dry grain before shaking it but you can still see some mycelium in there. This is a very slow culture and took 2 weeks to fully colonize a 60mm plate.

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This jar will take close to 3 weeks to colonize and im surely not going to put a lot of effort into this culture maybe fruit a bag, clone, and slant.

#18 Microbe

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Posted 07 November 2016 - 04:33 PM

Its been awhile since i shared my filter design so i figure might as well do it again.

I swear by these filters and they are very cheap to make.

I get micropore tape at CVS at the pharmacy, they keep it behind the counter and not in resale packaging but they hook me up with 6 rolls 2" wide for $4.00. This will last me several years.

I purchase the eazyfelt at hobby lobby. For a 12x14 sheet it costs me $ 1.07 after tax. There are different colors also but i prefer white so i can identify contamination growing on the filter, it has happened.

Eazyfelt is matted polyfill and when used in conjunction with micropore tape, i would put these up against SFD's.

I still use the 5 hole pattern as shown

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A take a piece of micropore tape and lay the felt as centred as possible.

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Place it over the holes and take my fingernail and push the tape down around the perimeter of the felt to make sure there is no gap between the felt and lid. In the PC the filters get wet but then the tape dries like freaking concrete firmly holding the felt in place.

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#19 CatsAndBats

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Posted 07 November 2016 - 04:36 PM

Its been awhile since i shared my filter design so i figure might as well do it again.

I swear by these filters and they are very cheap to make.

I get micropore tape at CVS at the pharmacy, they keep it behind the counter and not in resale packaging but they hook me up with 6 rolls 2" wide for $4.00. This will last me several years.

I purchase the eazyfelt at hobby lobby. For a 12x14 sheet it costs me $ 1.07 after tax. There are different colors also but i prefer white so i can identify contamination growing on the filter, it has happened.

Eazyfelt is matted polyfill and when used in conjunction with micropore tape, i would put these up against SFD's.

I still use the 5 hole pattern as shown

43632b7819ea4730ba3a3e3618eb74f7.jpg

A take a piece of micropore tape and lay the felt as centred as possible.

4692fb813f2f1a23c67d5e7b765a0a05.jpg

Place it over the holes and take my fingernail and push the tape down around the perimeter of the felt to make sure there is no gap between the felt and lid. In the PC the filters get wet but then the tape dries like freaking concrete firmly holding the felt in place.

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Fucking brilliant. I like the five hole design. When I run micropore through the PC I have different results though. You use rings under there?



#20 Microbe

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Posted 07 November 2016 - 04:58 PM

Once again i let my grain sit for a week in then PC and finally got around to inoculating them today. I also did some agar work to expand some inoculate.

These are all unidentified cultures and some of them are crazy and literally are growing out of the plates. Im back to using parafilm because i had money on my paypal account and couldnt think of anything else to buy. I am really beginning to enjoy using it though.

When increasing inoculate, i pour extremely thin plates. There is no need to pour thick if you are not going to store them for awhile. These plates were poured May 2015 and i was out of black food coloring

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There are 5 unknown cultures and i transferred using a new tool i wanted to try out. It still nichrome wire but its not a loop. It is a very thin single strand of wire that you attach to a special handle. This will give you pin point accuracy durung the selection process and extremely useful when isolating from a contamination.

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You can hardly see the transfer in the left plate but it is there. The one on the right is a little more noticeable.

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Here are the unknown cultures i am working with.

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The culture is growing on the underside of the lid.
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This was 2 separate cultures placed on opposite ends of the plate.
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Some pics of the myc growing on and around the lid, a few actually had the myc growing out and down the outer wall of the plate between the lid.

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Im going to use this in some plates, prepare the myc for some WBS. Going to stain it with black food coloring first before pcing it. Im not sure how it will hold up but the moisture content is very desirable.

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Ready for incubation!

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I felt very clumsy and my technique is off. It is going to take a few projects to get back in the groove!

I will post some progress in about a week. I will be surprised if i come out of this without a contamination.




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