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Cubensis Breeding: A Group Effort


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#41 Needles

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Posted 30 January 2017 - 02:55 AM

In your pictures they do not look to be evading each other or even retreating. The hyphae have certainly passed the natural zone and i speculate that each will halt or overrun the other. Break out one of your other bad ass scopes and capture the clamp connection.
Yeah you will.....


I have one slide that should be binding tomorrow. I have it sitting on my phase contrast microscope. It is very cool the way the mycelium looks...
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#42 CatsAndBats

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Posted 30 January 2017 - 08:41 AM

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You can play the lotto too.

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Edited by catattack, 30 January 2017 - 08:41 AM.

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#43 Microbe

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Posted 30 January 2017 - 03:42 PM

Wow! From all that you received the the infamous "666" recognition! You have been around much longer then I so you know thats celebrated around here. Im surprised hyphenation hasnt showed up with his devil tongue gif! Damn i pissed rught now! I need to go punch my fucking dog in the snout right now and tell her to stop eating poop. I will be back.
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No need to kick the dog lol it's all good...... Funny how our pets like to eat poop lol I would watch our jack eat horse poo then I would tell her to go give mommy a kiss lol.....
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Not to chamge the course of this thread but why do dogs eat poop? I really do want take some leather to my dog when she does this but i would never abuse her unless she bit one of us.

#44 CatsAndBats

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Posted 30 January 2017 - 03:50 PM

found here: http://www.dogpoopdi...-dogs-eat-poop/looks like they're selling something so take it with a grain of salt, but I like their first answer

 

 

 

5 Reasons Why Dogs Eat Poop

 

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Like humans, dogs each have their own motives for their individual behavior. Dogs eat poop for various reasons, based on their age, their training, their living conditions and their diet. There are basically 5 different classifications, or reasons that your dog may be eating poop.

 

Your Dog Was Born to Eat Poop!

Before dogs were domesticated they were scavengers, living off of whatever they could find. Dogs commonly fed on the waste of other animals (and other dogs) thousands of years ago. Poop eating may just be a remnant of dog history.

In certain situations, as with a newborn litter of puppies, eating poop is instinctual and completely normal. A mother with pups is wired to keep her den clean so as not to attract predators with scent cues. Thus, she quite often will clean up after her young by consuming their poop.

For households with multiple dogs there is often a pecking order of dominant and submissive roles. Submissive dogs will sometimes eat the stool of their dominant counterparts.

 

Dogs Are “A” Students

Dogs pick up things quickly and will often learn things that you don’t want them to. For instance, consider a dog that is punished for a housebreaking accident. If he is punished by having his nose rubbed in poop (which is absolutely not a good way to deal with the problem) he may try to “dispose of the evidence” the next time around.

If you clean up after your dog while he looks on, he may misunderstand your intent and try to copy your actions in some fashion by “picking up after himself”. Your dog might also see other dogs eating poop and learn the behavior from them.

For puppies, eating feces may simply be a learning experience. Puppies learn things by putting nearly everything that comes in front of them in their mouth. Most puppies will develop a distaste for poop in fairly short order. So, if your dog is a puppy, you can relax… chances are that they will change their behavior in due time. Just make sure you keep an eye on things and try to remove waste whenever possible so that your dog doesn’t develop bad habits.

 

Why You Should Take Your Poop Eating Dog to the Vet

If your dog eats poop, you should make sure it’s not because of a health issue. Some dogs will start eating poop when they aren’t absorbing enough nutrients, they have parasites, or they have issues with their pancreas. All coprophagic dogs should be examined by a veterinarian. Please read my other post on coprophagia and dog health.

Another, rather interesting phenomenon is when multiple dogs are in the same household and one gets sick, the healthy dog will sometimes eat the feces of the unhealthy dog. This may be an instinctual reaction to hide the weaker dog from “predators” much as a mother does with pups (see the section on instinct below).

 

Why Your Dog Sometimes Prefers Poop to Dog Food

A dog’s digestive system is dependent on a specific mix of enzymes to break down carbohydrates, proteins and fats. There is some evidence that suggests that dog digestive systems haven’t quite caught up to modern diets that include less animal protein and far more carbohydrates and plant proteins. Some veterinary nutritionists have suggested that dogs eat stool to replenish enzymes so that they are better prepared to digest their food.

There is also evidence that dogs that aren’t getting enough of certain nutrients will resort to eating poop. A lack of vitamin B is often said to be a cause of coprophagia.

Another common theory is that overfeeding a dog can lead to coprophagia. A dog that is overfed can’t absorb all of the nutrients in his food, and thus may try to “recycle” his nutrient rich waste.

 

Neglectful Parents

In many cases, a dog’s behavior can be linked directly to the owner’s behavior. Many dogs will eat stool simply for the attention that they get from their owner. Negative attention is still attention, and owners who scold their dogs for the behavior will quite often only reinforce it.

Dogs that are bored and lonely may play with and eat stool as a pastime. And, some dogs may resort to eating stool because they are not getting enough real food. If a dog’s living area is not kept clean, some dogs will resort to their own “housekeeping” efforts by eating stool.


Edited by catattack, 30 January 2017 - 03:53 PM.


#45 Needles

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Posted 31 January 2017 - 01:34 PM

Ok back on track, I believe I located strands of hyphae from both strains of mushrooms connecting.

Pardon the shakie lines I used a stylus and pad. Anyway the green line is H. marmoreus and the blue is H. tessulatus and the red arrow points to the connection. It almost looks like a drop of glue, I believe that is metabolic fluid. There is another spot towards the top that looks like another connection on the same strand. When working with photography like this there is very little depth of field or sharp focus so I did the best I could on this image.
image.jpeg

I am adding a micrograph of the leading edge of hyphae growing out looking for nutrients.
image.jpeg

I have learned a lot from this experiment and am getting ready to do it again. I will come back with a stained sample that may be easier to identify what I believe is metabolic fluid being secreted from the mycelium.
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