There hasn't been much going on in this part of the site for awhile so I thought I would share a experiment with you.
I have been working with storing cultures in sterile water for about four years now. I will start with a monoculture on agar.
When the culture has grown out on the plate the mycelium is scraped off
Once harvested the mycelium is added to sterile water and blended

after the mycelium has been shredded it is extracted with a sterile syringe then added to sterile bottles for storage

after the venders supply of bottles ran out I started looking at other options. I came across this septa bottle

it's 40ml, a little larger than I like but seems to work ok.
My syringes are cooling in the PC now so later tonight I will inoculate two quart jars of grain for each species.

most of these cultures are two years old but a couple are almost four now.