To get around both of these you sterilize a jar, tube , etc of distilled water with no GE hole and this will create a vaccum. Then you harvest the mycelium from a agar plate leaving behind the agar and blend it thank you needles for my blender lids and motor, then you draw into the syringe and when you inject the container with the sterile distilled water under vacuum, it pulls in the mycelium water, which also needs to be distilled as i forgot to mention, the contents will be pulled in by the vacuum.
Excellent thread, lovin' it.
How can you assure there are no nutrients available to the myc? There may be some agar present from the initial harvest. Perhaps wait some time (a few days, week?) with the suspension in the sterile water prior to refrigeration -> let the myc eat up any remaining nutrients. But . . . if done in a vacuum, then there is no O2 for burning the nutrients. Maybe nutrient and no O2 is bad (wondering if myc has any anaerobic metabolic options like with animals.
Or do we just assume that we did not pick up much agar with harvesting of the myc?
I have an idea and wonder what you think about it. I have a professional commercial vacuum chamber sealer that can seal liquids. I gets to a really high level of vacuum, like < 1 mm Hg, then seals under vacuum and better that what you will get with a steam seal in a jar. It seals with heat in tough plastic bags.
I know what you are thinking. It's not anything like a cheap vacuum sealer. This thing weighs 80# and is around $600 but, btw, is an awesome kitchen and lab tool you never knew you needed. Unlike an external vac sealer, it can do liquids. Anyway, getting off topic but this is a good intro to the machine.
The sealer bags are autoclave tolerant. I imagine you could 'clave some bags or just do a chemical sterilization, then blend and seal some myc and refrigerate. I'll take some pics and maybe do a demo for kicks. The myc would be at atmospheric pressure in a flexible bag but sans any gas in the bag or the liquid for that matter. The vacuum chamber extracts gas from the liquid as well to some extent (exp with the longer vac cycles available). And the bags are not gas permeable.
Can you give any references etc for preservation of myc under nutrient free medium and vacuum. Would be a cool but really lengthy (!) experiment to do - compare long term storage techniques and conditions. Could do 4 groups - Vacuum or not combined with nutrient or not.
Edited by raymycoto, 28 January 2018 - 05:29 PM.