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Reviving H20 suspended mycelium


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#81 Arathu

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Posted 14 February 2021 - 08:42 PM

Yeah like that....I have 2000 here waiting on me to put in the work.....spring is coming soon...

 

A


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#82 Needles

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Posted 21 February 2021 - 07:02 PM

Getting ready to store five new cultures....

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#83 Arathu

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Posted 21 February 2021 - 08:39 PM

Sweet! Needles is about to operate!

 

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#84 sandman

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Posted 22 February 2021 - 10:00 AM

go go gadget needles


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#85 jrh

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Posted 22 February 2021 - 10:05 AM

Wow, those are some serious needles. I never even imagined having glass syringes.


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#86 Needles

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Posted 22 February 2021 - 08:34 PM

There is some agar blended in and can get chunky. The needle is stainless steel, 3.5 inches long and a 13 gauge beveled tip. If anyone wants to start using this kind of equipment the tip of the needle should not touch anything. If it is pushed into a glass jar the chances of bending the point are great making it difficult to use on the next project. Glass syringes and stainless needles can be autoclaved and reusable. 


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#87 Needles

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Posted 16 November 2021 - 02:30 PM

last year I was able to revive a Azur culture that was blended and stored at room temp from 2012. It made it to a fruiting tote/tub then outdoors. Weather went from summer to almost winter here so all we got was a few pins. Just enough to start a clone and begin to acclimate and develop a northeast strain of azur. being a small amount of tissue to work with, my choice for agar was PDYA plus H202.Upon closer examination there was a tremendous amount of mycelium growing from the pins overnight. Its not to sure about the H202 agar yet but with time it should start to expand. I also found a couple of transparent worms living in the body of the pin. I will try to get a phase contrast micrograph of them if possible real soon..

 

 

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#88 Needles

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Posted 16 November 2021 - 05:49 PM

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Mycelium growth has been extremely aggressive from this tiny pin, that small larva has been covered plus better to not open the plate anyway. Plate choice was a 150mm glass Pyrex petri and sealed with laboratory tape. The close up was taken through the glass and hard to see but I circled a colony of bacteria that came with the transfer despite using hydrogen peroxide. There Is also a very aggressive mycelium growth on the agar already, I'm kind of nerves about it so will have to keep a eye on that.....

 

 

 

 

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#89 Arathu

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Posted 16 November 2021 - 08:08 PM

Those definitely don't look like they aborted......fuzzing up nicely.... as usual......excellent!

 

A


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