The basic premise is to use the hormones found in primordia to trigger faster and more controlled pin sets. Using these principles, one could possibly control where he or she wants the surface of the colonized substrate to pin. I do not have access to a lab or equipment, so as we amateur cultivators tend to do, I will improvise.
The tek I am following comes from “Organic Mushroom Farming and Mycoremidiation” by Tradd Cotter and a small amount from good old TMC. Tradd Cotter called for primordia to be harvested, cleaned, surface sterilized with ethanol, blended in sterile water to make a slurry, vacuum filtered, 5 minutes in a centrifuge, sterilized using a syringe filter, then finally diluted and applied to a fully colonized substrate.
I have decided to keep it simple at first and only work with agar. If I get any results, I will move to bulk grows. My plan is to grow out several agar jars and use roughly half for a control, i.e. no hormone treatment, and the other half, I will apply tiny amounts of hormone extract in a calculated pattern on the agar surface.
I do not own a vacuum filter system, Buchner funnel, centrifuge, or syringe filters, so here comes the improvisation.
My homemade vacuum filter:
It works okay, a little slower than a lab grade system would, but it did the trick in my test trials with some LI I didn’t mind wasting.
I read about someone publishing an article where he used an egg beater to separate plasma from blood. I did not have one, so I decided to use the beater attachment on my Kitchen Aid. Hopefully it should work but I will spin on high speed for 10 minutes, not 5. It took that long to separate mycelium particulates from the same LI in my equipment test trials.
My make shift centrifuge:
And the syringe filter is the easy part. I found some for pretty cheap on Amazon. Ordered as of yesterday.
Below is a pic of my slant:
Notice how matted it looks, but only after 12 hours out of cold storage there are signs of new growth emanating from previously un-colonized agar. I hope it can be seen with my cheap camera (lower middle of pic). It goes to show how resilient mycelium truly is.
Wish me luck here and if anything good or promising comes from this, I will most definitely update as I go.
Edit: I just realized after I posted this, it may need to be in the Mycolab or Mad scientist forum? If so I apologize and any mod feel free to move.
Edited by peacefrog, 08 January 2017 - 09:39 AM.